Generator

Part:BBa_I737021:Design

Designed by: Vladislav Lavrovsky   Group: iGEM07_Calgary   (2007-10-26)


Constitutively expressed Agarase part.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1943
    Illegal NheI site found at 188
    Illegal NheI site found at 983
    Illegal PstI site found at 366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1943
    Illegal XhoI site found at 2046
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
    Illegal AgeI site found at 450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1401
    Illegal SapI.rc site found at 1799
    Illegal SapI.rc site found at 1877


Design Notes

There are EcorI and PstI sites. This limits construction. Future versions will be mutated by site-directed mutagenesis. The flanks of the gene were included for the signal peptide. Agarase is exported in the source organism using the TAT export system with e. coli K12 has, unfortunately so far e. coli dont degrade agar when transformed with this part. We are not sure whether it is a problem with expression or export.


Source

Cloned from Pseudoalteromonas atlantica T6C.

References